GENERAL INFORMATION 
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1. Dataset title: Dataset zebrafish and its interaction with NNV


2. Authors: M. Carmen Alonso, Esther Garcia-Rosado, Julia Bejar, Patricia Moreno


3. Author contact information: M. Carmen Alonso (mdalonso@uma.es)


METHODOLOGICAL INFORMATION 
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1. Description of the methods for collection/generation of data: 

Itramuscular injection of NNV isolate SpDl-IAusc965.09 (DlNNV, RGNNV genotype) in zebrafish juveniles. Disease progression was observed at several times post-infection.


2. Data processing methods

- Intramuscular injection

3. Software or instruments needed to interpret the data: Not applicable

4. Standards and calibration information, if appropriate: not applicable

5. Environmental or experimental conditions:
- Virus propagation and cell culture: E-11 cells were grown in Leibovitz L-15 medium (Gibco, Paisley, UK) supplemented with 10% foetal bovine serum (FBS, Gibco, Paisley, UK), 100 unit/mL penicillin and 10 mg/mL streptomycin (Sigma) at 25 °C until confluence. 
The viral isolates SpDl-IAusc965.09 (DlNNV, RGNNV genotype) were propagated in E-11 cell in L-15 medium supplemented with 2% FBS, 100 units/mL penicillin and 10 mg/mL streptomycin at 25 ºC 
-Fish maintenance: fish were maintained under the following conditions for 30 days: 24 ± 0.5 ºC, pH 7.5 ± 0.5, salinity 38 ± 1 ppt, oxygen 6 ± 1 mg/l, NO2 < 3 mg/l, light 12 h.

FILE OVERVIEW 
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The dataset is distributed in 1 files in .xlsx format. This provides information on:

- Challenge RGNNV zebrafish 12-06-25 (.xlsx)

	

DATA-SPECIFIC INFORMATION:
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1. Name File: Challenge RGNNV zebrafish 12-06-25 (.xlsx)

1.1. Variables list:
Variable name: Injection
Description: Methodological information of the intramuscular injection of zebrafish juveniles with RGNNV, with weight, size and volume inoculated.
Units of measure or value labels: for weight the unit was mg, for size mm, and for volumen the unit was ul.

1.2. Codes or symbols: 
mg :miligrams
mm: milimeters
ul: microliters
	
1.3. Special formats or abbreviations used: Not applicable



MORE INFORMATION

These data derived from the project "Interplay between nodavirus virulence and innate response in sea bass and sea bream (INTERNODASS)" (Proyectos de I+D+i Retos Investigación, PID2020-115954RB-I00)
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